This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
这份极致的艺术表达并未给塔可夫斯基带来预期中的荣誉。在当届戛纳电影节上,塔可夫斯基最终与布列松共同获得最佳导演奖,与期盼多年的金棕榈奖失之交臂。塔可夫斯基在日记中详细记录了电影节上的种种遭遇,他认定这场惜败是苏联当局的刻意操作,是叶尔马什任命苏联导演邦达尔丘克担任评委一手策划的结果:“邦达尔丘克一直在跟《乡愁》较劲。显然,他被派到戛纳就是为了否定这部电影。”字里行间满是失望和愤怒。也正是这场戛纳的失意,击碎了他回国继续电影创作的信心,让他在日记中写下了那个艰难的决定:永远不再返回苏联。
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